By completing this project, we determined that the mixture with the highest concentration of casein was most effective in forming fibers; however, after testing the effect of water on the fibers, we found that many had disintegrated while the fibers of mixtures with lower percentages of casein remained intact. We had originally attributed this result to insufficient drying time, although after one week, the broken fibers in both samples of the 2:1 mixture were unchanged. Sources of error in this experiment are: failing to mix the earlier casein-PEO solutions (1:1, 1:4) fully, using ammonia containing chellating agent for the earliest attempts, and inconsistent use of a foil-covered slide to collect fibers during electrospinning.
"Ultra-high surface fibrous membranes from electrospinning of natural proteins: casein and lipase enzyme" by Jiangbing Xie, You-Lo Hsieh: http://www.springerlink.com/content/m738q18413642454/fulltext.pdf?page=1
Special thanks to Dr. Hubbard and KC